ADVANCEMENT REPORT ON POPULATION GENETIC STUDY OF BROWN HARE POPULATIONS IN CENTRAL AND EASTERN EUROPEAN REGION
Abstract
The brown hare is present all over Europe from Spain to Asia Minor and is an important game species which makes it a reasonable subject for population genetic investigations. The purpose of our study is to gather data on the genetic diversity of Central and Eastern European brown hare populations with the aim of revealing the genetic history of the species in the studied region, from which data are scarce despite the fact that the species is well-studied on the whole continent. In our research mtDNA cytb and D-loop regions along with the MC1R and ASIP genes are studied. Samples from Hungary (n=14) and Serbia (n=36) have already been sequenced and analysed for the D-loop region. Based on a 411 bp mtDNA D-loop alignment we have found a high level of haplotype diversity (Hd=0.958) with an overall 30 haplotypes, and the nucleotide diversity of π= 0.0167. Our first results show high genetic diversity for the brown hare in the studied region and indicate genetic distinction among the studied populations although these results need confirmation by further studies.
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Alves P.C., Ferrand N., Suchentrunk F., Harris D.J. (2003) Ancient introgression of Lepus timidus mtDNA into L. granatensis and L. europaeus in the Iberian Peninsula, Molecular Phylogenetics and Evolution, 27 pp. 70–80.
Alves P.C., Melo-Ferreira J., Branco M., Suchentrunk F., Ferrand N., Harris D.J. (2008) Evidence for genetic similarity of two allopatric European hares (Lepus corsicanus and L. castroviejoi) inferred from nuclear DNA sequences, Molecular Phylogenetics and Evolution, 46 pp. 1191–1197.
Andersen L.W., Fredsted T., Wincentz T., Pertoldi C. (2009) Brown hares on the edge: Genetic population structure of the Danish brown hare, Acta Theriologica, 54 (2), pp. 97–110.
Antoniou A., Magoulas A., Platis P., Kotoulas G. (2013) Assessing the genetic landscape of a contact zone: the case of European hare in northeastern Greece, Genetica, 141, pp. 23–40.
Djan M.R., Vapa L.B., Obreht D.R., Vapa M.M., Šelmić V.R. (2004) On gene pool divergence of the brown hare (Lepus europaeus, Pallas) in Vojvodina, Proc. Nat. Sci. Matica Srpska Novi Sad, 107, pp. 13-20.
Djan M., Obreht D., Vapa L. (2006) Polymorphism of mtDNA regions in brown hare (Lepus europaeus) populations from Vojvodina (Serbia and Montenegro), European Journal of Wildlife Research, 52, pp. 288–291.
Endler Z., Jezierski W. (1995) The structure of habitats and the diet of hares. In: Hare – International Symposium, Czempiń 1992., pp. 231–245. Polski Związek Łowiecki, Warszawa. (In Polish)
Fickel J., Hauffe H.C., Pecchioli E., Soriguer R., Vapa L., Pitra C. (2008) Cladogenesis of the European brown hare (Lepus europaeus Pallas, 1778), European Journal of Wildlife Research, 54, pp. 495–510.
Hartl G.B., Suchentrunk F., Nadlinger K., Willing R. (1993) An integrative analysis of genetic differentiation in the brown hare Lepus europaeus based on morphology, allozymes, and mitochondrial DNA, Acta Theriologica, 38(2), pp. 33–57.
Hasegawa M, Kishino H, Yano T (1985) Dating of human-ape splitting by a molecular clock of mitochondrial DNA, Journal of Molecular Evolution, 22(2), 160–174.
Hewitt G.M. (1999) Postglacial re-colonization of European biota. Biological Journal of the Linnean Society, 68, pp. 87–112.
Kasapidis P., Suchentrunk F., Magoulas A., Kotoulas G. (2005) The shaping of mitochondrial DNA phylogeographic patterns of the brown hare (Lepus europaeus) under the combined influence of Late Pleistocene climatic fluctuations and anthropogenic translocations, Molecular Phylogenetics and Evolution, 34, pp. 55–66.
Librado, P. and Rozas, J. (2009) DnaSP v5: A software for comprehensive analysis of DNA polymorphism data, Bioinformatics, 25, pp. 1451-1452.
Mamuris Z., Sfougaris A.I., Stamatis C. (2001) Genetic structure of Greek brown hare (Lepus europaeus) populations as revealed by mtDBNA RFLP-PCR analysis: Implications for conserving genetic diversity, Biological Conservation, 101(2), pp. 187-196.
Mamuris Z., Moutou K.A., Stamatis C., Sarafidou Th., Suchentrunk F. (2010) Y DNA and mitochondrial lineages in European and Asian populations of the brown hare (Lepus europaeus), Mammalian Biology, 75, pp. 233–242.
Mengoni C. (2011): Phylogeny and genetic diversity of Italian species of hares (genus Lepus). Ph.D. thesis. University of Bologna, Italy.
Nei M. (1987) Molecular Evolutionary Genetics. Columbia University Press, New York.
Nei M. and J. C. MILLER (1990) A simple method for estimating average number of nucleotide substitutions within and between populations from restriction data. Genetics 125: 873-879.
Panek M., Kamieniarz R. (1999) Relationships between density of Brown hare (Lepus europaeus) and landscape structure in Poland in years 1981-95, Acta Theriologica, 44, pp. 67-75.
Pierpaoli M., Riga F., Trocchi V., Randi E. (1999) Species distinction and evolutionary relationships of the Italian hare (Lepus corsicanus) as described by mitochondrial DNA sequencing, Molecular Ecology, 8, pp. 1805 –1817.
Rozas, J., Sánchez-DelBarrio, J. C., Messeguer, X. and Rozas, R. (2003) DnaSP, DNA polymorphism analyses by the coalescent and other methods, Bioinformatics, 19 pp. 2496-2497.
Stamatis C., Suchentrunk F., Moutou K. A., Giacometti M., Haerer G., Djan M., Vapa L., Vukovic M., Tvrtković N., Sert H., Alves P. C., Mamuris Z. (2009) Phylogeography of the brown hare (Lepus europaeus) in Europe: a legacy of south-eastern Mediterranean refugia?, Journal of Biogeography, 36, pp. 515–528.
Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0., Molecular Biology and Evolution, 30, pp. 2725-2729.
Tapper S.C., Barnes R.F.W. (1986) Influence of farming practice on the ecology of the brown hare (Lepus europaeus), Journal of Applied Ecology, 23, pp. 39–52.
Vapa L., Obreht D., Vapa M., Selmic V. (2002) Genetic variability in brown hare (Lepus europaeus) populations in Yugoslavia. Zeitschrift Fur Jagdwissenschaft 48, pp. 261–266.
Vapa L., Djan M., Obreht D., Hammer S., Suchentrunk F. (2007) Allozyme variability of brown hares (Lepus europaeus) from the Vojvodina (Serbia), compared to central and south eastern European populations, Acta Zoologica Academiae Scientiarum Hungaricae, 53, pp. 75-87.
DOI: http://dx.doi.org/10.15679/bjwr.v3i1.32
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ISSN: 2335-0113
Publisher: Visio Mundi Academic Media Group
This journal is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.